Isolation of Entomopathogenic Nematode-Bacteria Complex with a Potential for Use as a Biological Insecticide in Nigeria
Keywords:
Galleria mellonella larvae, entomopathogenic nematode, SteinernemaAbstract
The larvae of G. mellonella obtained from honey combs were successfully grown to adult on newly developed artificial medium in the laboratory. Generations of the moth were grown and maintained on the artificial medium. The larvae were used as bait to trap entomopathogenic nematodes from the soil. The nematodes isolated were identified as Steinernema species based on their morphology. The pathogenicity of the nematodes is tested after storing them in water for three and six months respectively. It is observed that the ability of the nematodes to kill the larvae reduced with increase in the period of storage. Nematodes stored for six months killed only 20% of larvae treated while that stored for three months killed 40% of larvae treated. Death of the larvae occurred within 24 hours for freshly harvested infective juvenile nematodes while it took 72 hours for nematodes stored for three months and 168 hours (7days) for those stored for 6 months. The nematode-bacteria complex isolated has a potential for use as a biological insecticide.
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