Effects of Dietary Advanced Lipid Oxidation End-products on Colitis Healing in Albino Rats
Keywords:
Lipid oxidation, Colitis, Albino rats, HealingAbstract
This Experiment is undertaken to investigate the effects of dietary advanced lipid oxidation endproducts on colitis healing in albino rats. 45 albino rats (divided into 3 groups - control, low dose and high dose ALE groups) of average weight of 200g were used for this study. Colitis was induced in all groups using 6% acetic acid. The low and high doses were fed with 7.5g and 15g ALE respectively for 20 days.The control animals ate normal rat chow. The stools of all animals were scored according to the scale of Masonobu et al (2002) for 20 days. On days 7, 14 and 20 three animals were sacrificed from each group and 8cm of the colon was cut out for weight measurement and gross morphological scoring.The results show that on day 6 colitis scores were 0.63 ± 0.03 (control), 0.80 ± 0.03(low dose),0.73 +_ 0.03(high dose). On day 20, 0.33 ± 0.03 (control), 0.50 ± 0.03 (low dose), 0.50 ± 0.02 (high dose). ALE reduced colitis healing rate compared to the control. This study concludes that ALE aggravated acetic-acid induced colitis in albino rats.
References
Addis, P. B. (1986). Occurrence of lipid oxidation products in foods. Food Chem. Toxicol. 24. 1021-1030.
Beutin, L. (2006). Emerging enterohaemorrhagic Escherichia Coli, causes and effects of the rise of a human pathogen. J. Vet. Med. B. Infect. Dis. Vet. Public Health 53 (7): 299 - 305.
Chang S. S.; Peterson R. J. and Ho C. T. (1978). Chemical reactions involved in the deep- fat frying of foods, J.AM. Oil Chem Soc., 56,718-727.
Cohn, J. (2003). Oxidized fat in the diet postprandial lipaemia and cardiovascular disease. Curr. Opin. Lipidol 13, 19-24.
Franke,l. E. (1998). Lipid oxidation, the oil press, Dundee. Gorelik S., Lipidot T., Shaham I. and Granit R. (2005). Lipid peroxidation and coupled vitamin oxidation in simulated and human gastric fluid inhibited by dietary polyphenols: health implications. Journ. Agric. Food Chem. 53, 3397-3402.
Hager H. H., Medany A. E., Eter E. E., Arafa M. and Eur, J. (2007). Pharmacol., 554: 69- 77 Jagtap A. G., Shirke S. S. and Phadke, A. S. J. (2004). Ethnopharmacol, 90: 195-204
Kanner, J. (1994). Oxidative processes in meat and meat products: Quality implications, Meat Sci. 36, 169-189.
Kanner, J. (2007). Dietary advanced lipid oxidation endproducts are risk factors to human health. Mol. Nutr. Food Res. 51, 1094 - 1101.
Kubow, S. (1992). Routes of formation and toxic consequences of lipid oxidation products in foods. Free Radic Biol. Med., 12, 63-81.
Lemaire-Ewing S.; Prunet C.; Montange T.; and Vejux, A. (2005). Comparison of the cytotoxic, prooxidant and pro-inflammatory characteristics of different oxysterols. Cell. Biol. Toxicol 21, 97 - 114.
Masonobu F.; Osamu K.; Yoshio A.; Akira A.; Kehchi M.; Kohsuke T.; Atsuchi T.; Michio S. Yoshihide F.; Masanuchi F.; Yoshiaki M.; and Tadao, B. (2002). Prebiotic treatment of experimental colitis with germinated barley food stuff: A comparison with probiotic or antibiotic treatment. Int J. Mol Med. 9: 65 - 70
Romano C., Famiani A., Gallizzi R., Comito D., Ferrau V. and Rossi P. (2008). Indeterminate colitis: a distinctive clinical pattern of inflammatory bowel disease in children. Pediatrics 122 (6), 1278-81.
