Morphological Studies Of The Effect Of Colchicine And Paradichlorobenzene On Tomato (Lycopersicon Esculentum)
Keywords:
Colchicine, Tomato,, ParadichlorobenzeneAbstract
The seeds of tomato (Lycopersicon esculentum L.) variety Roma VF obtained from Institute of Agricultural Research (IAR), Ahmadu Bello University Zaria, were treated with Colchicine and Paradichlorobenzene with the aim of determining the effectiveness of the mutagens in the production of variations in Tomato. Highly significant difference were observed for germination percentage, number of leaves, and height at maturity. Seedling height, number of branches and germination percentage decrease with increase in concentration of Colchicine and there was increase in leaf length, height at maturity with increase in concentration. While there was highly significant difference in germination percentage, number of leaves, and significant difference in leaf length and height at maturity for Paradichlorobenzene. There was decrease in all the traits that were studied; however, there was increase in seedling height, leaf length, and number of branches with increase in concentration. Paradichlorobenzene showed the highest effect in seedling height, germination percentage at both 5 and 8 days respectively. While colchicines showed the highest effect only in number of branches.Therefore, the use of 1mm and 4mm Colchicine and Paradichlorobenzene is recommended for inducing mutation to create variability and thus increase the possibility of isolating beneficial mutants.
References
Abieu, F. (1988). Using mutation in breeding of pure lines of maize. Fundamental Agriculture Tropical, 213-220.
Acquaah, G.. (2002). Horticulture principles and practices. New Jersey:
Adamu A. K. (1997) Preliminary Investigation on the influence of Gamma rays Cobalt 60 and Thermal radiation and tiller, ear production, maturity and Male sterility in popcorn (Zea mays) cultivars (praecox ). Nigerian Journal of Botany, 10, 51-59.
Adamu, A. K. (2004). Gamma -Rays (60Co) and thermal neutrons induced Mutants in popcorn (Zea mays var. Praecox sturt). Nigerian Journal of Scientific Research, 4, 52-63.
Adamu, A. K., Chung, S.S and Abubakar, A. (2004). The effects of ionization radiation (gamma rays) on tomato (Lycopersicon esculentum L.). Nigerian Journal of Experimental and Applied Biology, 5(2), 185-193.
Ahloowalia, B. S. and Maluszynski, M. (2001). Induced mutation : A new Paradigm in plant breeding. Eucalytica, 118, 167-173.
Khalitkar, A. S. and Bhatia, C. R. (1975). Synergistic effect of combined treatment of gamma radiation and ethyl methane sulfonate in Barley. Radioactive Botany, 15:223-229.
Krishna (2007). Tomato genume. News comell. education. Retrieved on 20 December 2008.
Mensah, J. K. and Obadoni, B. (2007). Effects of sodium azide on yield parameters of groundnut (Arachis hypogea L.) African Journal of Biotechnology, 6(6), 668-671.
Nilan, R. A. (1973). Increasing the effectiveness, efficiency and specificity of mutation induction in flowering plants. In Genes, Enzymes and populations A. Srb. Plenum Publication Coop. pp 205-222
Rauts R. N., Jain H. K. and Gupta R. (1971). Radiation induced photosensitive mutants of Cotton. Current Science, 14, 383-384.
Sasi A., Dhanayel D. and Pavadai P. (2005). Effect of chemical mutagenesis on bhendi [Abelmoschus esculentus (L.) Moench var. MDU-1]. Research On Crops, 6 (2), 300-306.
Smithsonianmag (2008). http:www.smithsonianmag.com/science-nature/ passion-For-tomatoes P.57
Takayi, Y. and Rahman, S. H. (1995). Variation of Different fatty acids in Mutants in comparison with Natural soybean varieties. Bulletin of the Faculty of Agriculture, Soga University, 79:23-27.
